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產品名稱:EPTOMICS代理Atg12 (ATG12) antibody Eptomics抗體供應商

產品型號:Eptomics抗體3473-1

產品報價:

產品特點:Atg12 (ATG12) antibody
Recommended Applications

WB, IHC, ICC, IP, FC

Eptomics抗體3473-1EPTOMICS代理Atg12 (ATG12) antibody Eptomics抗體供應商的詳細資料:

EPTOMICS抗體代理Atg12 (ATG12) antibody Eptomics抗體供應商3473-1

Rabbit Monoclonal Antibody

 

Description

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy (1). It is essential for Atg12 conjugation, and the ATG12-ATG5 conjugate is necessary to form autophagic vesicles (2). The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required (3).

Recommended Applications

WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors

WB: 1:1,000-10,000
IHC: 1:50-100
ICC: 1:100-250
IP: 1:10-100
FC: 1:10-100
 

Species Reactivity *

Human

*Species cross-reactivity is based on WB analysis


Products Data

Western blot analysis on (A) THP-1, (B) HepG2, (C) Raji, and (D) fetal kidney lysates using anti-Atg12 RabMAb (cat. #3473-1). Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Atg12 RabMAb (cat. #3473-1). Western blot analysis on (A) Atg12 transfected 293T and (B) 293T lysates using anti-Atg12 RabMAb (cat. #3473-1).


Specificity

A synthetic peptide corresponding to residues in human Atg12 was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ATG12 antibody, APG12 antibody, APG12L antibody, FBR93 antibody, HAPG12 antibody, Ubiquitin-like protein ATG12 antibody, Autophagy-related protein 12 antibody

Description References

1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.
2. Phillips AR et al. Genetics. 2008 Mar;178(3):1339-53.
3. Williams RA et al. Autophagy. 2009 Feb;5(2):159-72.

 

 

 

 

Product Name Catalog #
Goat Anti-Rabbit IgG (H+L) HRP 3053-1
Goat Anti-Rabbit IgG polymerized HRP 3051-1
Cell Lysis Buffer 3500-1
Goat Anti-Rabbit IgG HRP Antibody 3056-1
Goat Anti-Rabbit IgG DyLight® 405 3063-1
Goat Anti-Rabbit IgG DyLight® 488 3064-1
Goat Anti-Rabbit IgG DyLight® 550 3065-1
Goat Anti-Rabbit IgG DyLight® 594 3066-1
Goat Anti-Rabbit IgG DyLight® 633 3067-1
Goat Anti-Rabbit IgG DyLight® 650 3068-1

 

 

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